The main purpose of the Routine Laboratory is to provide diagnostic histology services. Demonstration for medical student and training MLS students , research , occasional before sending all. The Routine Laboratory is made up of: Reception : where the specimen is received and registered .Then processed and Blocked out of tissue for sectioning.Urgent Frozen sections for immediate diagnosis and other frozen sections for other Purposes are done here. Microtome sectioning: where the paraffin blocks are sectioned. Staining and mounting section: where sections are stained and mounted. Theroutine method is haematoxylin and eosin “H&E“. Checking and sorting out of the sections with the correct request forms before sending all to the pathologist for reporting. Health & Safety Regard all specimens as potentially infective. Therefore, universal precation should be used in handling the specimens. All routine histology material must be received in a tightly closed specimen container with 10% formalin and placed in a clear plastic bag. Unfixed tissue maybe received in laboratory (e.g. frozen section, kidney biopsy) and should be considered a special risk and handled as a high risk specimen. Disposables gloves, masks and aprons must be worn when handling infective specimens. After dissection, knives and forceps etc should be disinfected in Cidex and the bench cleaned with phenolic disinfectant. All contaminated parts of a cryostat used for sectioning infective specimens-chuck, brush, microtome knife, etc should be placed in 2% Glutaraldehyde for 24 hours. An open bowl of formaldehyde is placed in the cryostat chamber the lid is closed and the instrument is shut off for 24 hours. The instrument and parts may then be safely cleaned in the usual manner. Waste alcohol and xylene used for processing tissues must be stored in drums in the store until removed by the waste company for disposal. Alcohol, xylol, and other flammables for use in the laboratory must be stored in Inflammable Stores or cupboards away from the main laboratory. Only minimum amounts of these materials should be in the laboratory at any given time. Caustic, toxic and other harmful reagents for use in the laboratory must be stored in special desig-nated and well ventilated rooms. Bottles of acids must be stored as close to the floor as possible in case of breakages. All corrosive and explosive reagents should be stored in in explosive and corrosive proof cabinet. Preparation of stains and reagents especially whose containing acids must be carried out in fume cupboards to prevent inhalation of fumes. All stains and all other chemicals used for dehydrating and clearing of slides must be covered at all times to prevent evaporation. Fume hoods should be used for mounting or coversliping of slides. Buckets of sand should be kept handy for containment of spillages prior to their being mopped. Wear appropriate protective clothing while dealing with spillages. Only minimum amounts of inflammables (25 liters each of Xylene, 74 OP alcohol and 66 OP alcohol) can kept in laboratory at any one time. All toxic and volatile reagents and chemicals should be handled in a fume hood. Care must be exercised in handling liquid Nitrogen. Face masks, gloves and special shoes must worn when handling this material. Liquid nitrogen must always be stored in separate rooms away from the main laboratory and properly covered. Microtome knives used for section cutting must never be left on the microtomes after sectioning. They should be put away in their protective boxes and stored away. Disposable blades should also be removed from micro- tomes and disposed of. Treat microtome knives with care and respect. They could cause severe injury if handled carelessly.