Cellular and Molecular Biology

Genetic Analyzers

Model: ABI 3130xl Genetic Analyzers

Make: Applied Biosystems

Features

  • Auto loading of samples performed from two microtiter plates; either 96- or 384-well format
  • Arrays with sixteen capillaries in four different lengths: 22, 36, 50, and 80 cm.
  • Automated delivery system for POP-4, POP-6, or POP-7 polymers
  • Argon laser with primary excitations at 488 and 515 nm
  • Detection of up to 5 dyes for sequencing and fragment analysis
  • CCD detection technology and spectrograph for color separation
  • Simultaneous dual-side illumination detection system to maximize signal uniformity and sensitivity
  • Software for DNA sequencing/base calling (Sequencing Analysis v5.2) and for fragment analysis of microsatellites, SNP, AFLP and LOH (GeneMapper® v4.0) as well as comparative sequencing, mutation detection, and detection of heterozygote insertions and deletions (SeqScape® v2.5).

 


Flow Cytometer (FCM)

Model: FACS Aria III Flow Cytometer

Make: Becton Dickinson (USA)

Features

  1. Two excitation lasers:
  • Blue (488 nm) Coherent Sapphire solid state; 13mW- 20mW
  • Red (633 nm) JDS Uniphase He-Ne air cooled; 10mW-20mW
  1. 10 measurement channels with following filters:
  • FSC, 488/10
  • SSC,  488/10
  • FL1,  FITC,  530/30, 502LP
  • FL2,  PE,  585/42, 556LP
  • FL3,  PE-Texas Red, 616/23, 610LP
  • FL4,  PerCP-Cy5-5,  695/40, 655LP
  • FL5,  PE-Cy7 780/60 ,735LP
  • FL6,  APC , 660/20
  • FL7,  APC-H7,  730/45, 685LP
  • FL8,  APC-Cy7,  780/60, 755LP
  1. Cell Sorting:
    • Sort up to four populations simultaneously
    • Sort rates up to 25,000 cells per second
    • Sort purities > 98%
  2. The sorted cells are collected in a variety tubes
  • 15ml flacon tubes for 2-way sort
  • 5ml FACS tubes for 4-way sort
  • 1.5ml and 1.0ml microcentrifuge tubes
  • 96-well plates
  • Microscope slides
  1. Data Analysis
  • FACSDiva software Version 6.1.3 is used in FACS Aria III for operating the instrument and analyzing the data