Chromatography & Mass Spectrometry

Gas Chromatography-Mass Spectrometry(GC-MS)

Model: Quattro micro GC/MS

Make: Waters Micromass MS Technologies

Features:

  • The Waters Micromass Quattro micro GC is a tendem quadrapole mass spectrometer equipped with Electron Impact (EI) & Chemical Ionization (CI+/CI-) Source.
  • It utilizes a dedicated Electron impact source optimized for performance with an easily accessible outer source containing the focus lens assembly and source heater. The measured signal-to-noise ratio obtained from the mass chromatogram of m/z 272 generated from repetitive scans at 300 amu / sec on injection of 1 pg of octafluronapthalene on to a capillary column will be greater than 10:1.
  • Chemical Ionization (CI+/CI-) is performed using a dedicated cool CI source, enabling low temperature operation down to 120ºC, fitted with a rhenium filament. Also includes an electronic mass flow controller for regulation of the CI reagent gas that can be set from Masslynx Software.
  • Resolution : Measured using the 614 m/z ion from Heptacosa, the resolution obtained will be greater than 1500(FWHH).
  • Sample Introduction: An Agilent 6890 Gas Chromatography is connected to the MS detector.

 

Gas Chromatography-Flame Ionization Detector (GC-FID-HSS)

Model: 7890A-7697A

Make: Agilent

  • Agilent 7890 with FID & Headspace: The Agilent/HP 7890 series GC features electronic pneumatic control of all gas pressures and flows. On board sensors automatically compensate for ambient temperature changes and barometric pressure differences to routinely achieve more accurate and reproducible results. By providing stable results, EPC reduces re-calibration frequency and improves laboratory productivity.

Features:

  • Single Split/Splitless Injector
  • Single Flame Ionization Detector
  • Electronic Pressure Control on detector and injector
  • HPIB Communications
  • This Agilent/HP 7890 GC with FID comes fully refurbished and tested at GenTech Scientific.
  • NEW GC Headspace Sampler:The high performance, gas-tight heated syringe is a simple and robust system. It eliminates the dead volume and absorption effects, typical of sample loops and transfer lines, which can also impede their detection at very low levels. The syringe only concept allows for sequential injections, even with samples characterized by highly dissimilar features. Even the most chemically active compounds can be analysed, without needing to change any of the sample pathways.

Features:

  • Compatible with ChemStation
  • Comparable to Agilent & CTC samplers
  • Easy to use
  • Lowest total cost of ownership in the industry
  • GC Mounting Kit & Cables
  • Touch screen
  • Orbital shaking capability
  • LAN and TTL Electrical Control

 

Inductive Coupled Plasma Optical Emission Spectrometer (ICP-OES)

Model: 8000 DV

Make: Perkin Elmer

Features:

  • Double spectrometer- The Optima 8000 ICP-OES uses a unique double-spectrometer optical system. This design results in a high-speed, high light-throughput optical system offering excellent resolution, all in a compact system. The sealed optical system can be purged with nitrogen for low UV (165-190 nm) performance.
  • Echelle spectrometer - The high-dispersion echelle spectrometer has a focal length of 0.3 meter and a Stigmatic Littrow configuration. The echelle grating used for dispersion has 79 lines/mm with a blaze angle of 63.4 degrees.
  • Parameters- The spectral range is 165-900 nm with resolution of < 0.009 nm @ 200 nm.
  • Plasma viewing- The system includes complete dual-viewing optics under computer and software control. Any wavelength can be used in the radial, axial or mixed viewing modes in a single method. With the patented dual-view capabilities of the Optima 8000 system, viewing of the plasma is accomplished by computer control of a mirror located in the optical path and allows selection of axial or radial view and adjustment of the plasma viewing in both the vertical and horizontal planes.
  • Detector- The UV-sensitive, dual backside-illuminated Charge-Coupled Device (CCD) array detector is cooled directly using a single-stage integrated Peltier cooler operated at approximately -8 °C. The detector has two photosensitive segments containing 176 by 128 pixels. One section is used for analytical measurements, and the other is a wavelength reference section. The CCD-array detector collects both the analyte spectra and the nearby background spectra, allowing for simultaneous background correction and providing improved precision and analytical speed.
  • Dynamic Wavelength Stabilization - The wavelength reference section of the detector monitors a full wavelength neon spectrum, creating a dynamic wavelength scale used to actively correct wavelength positions. The resulting wavelength accuracy and reproducibility allows direct ‘on-peak’ measurements rather than time-consuming peak-search methods used in sequential ICP systems.
  • RF generator -The Optima 8000 features a fourth-generation 40 MHz, free-running solid-state RF generator, adjustable from 750 to 1500 watts, in 1 watt increments. The power efficiency is greater than 81% with < 0.1% variation in output power stability. True Power Control maintains the plasma power at the set point, even when changing sample matrices. The compact RF supply meets all FCC certification requirements for RF emission (Part 18 of FCC rules and regulations) and complies with EC and VDE 0871 Class B requirements.
  • Ignition and power control -Plasma ignition is computer-controlled and totally automated. The plasma can be ignited automatically at a user-determined time and turned off automatically after an analysis.
  • Safety interlocks- For user safety and system protection, the system constantly monitors water flow, shear gas pressure, argon pressures, sample-compartment door closure and plasma stability, and displays the interlock status on the computer screen as graphic symbols. If an interlock is interrupted, the plasma will immediately and safely shut down.
  •  Cooling water- A water-recirculating cooling system is required, with approximately 4 L/min flow capacity at 310 to 550 kPa and a temperature between 15 °C and 25 °C.
  • Argon flow -Computer-controlled solenoid valves are used to regulate the flow automatically within the range of 0-20 L/min in 1 L/min increments for plasma argon and 0 to 2.0 L/min in 0.1 L/min increments for auxiliary argon. A mass-flow controller is supplied with all systems for the nebulizer argon flow and is variable between 0 and 2.0 L/min in 0.01 L/min increments.
  •  Shear gas -A compressed-air shear gas (18-25 L/min) is used to remove the plasma tail from the optical path, minimizing interferences and extending the dynamic range. The shear gas design offers a maintenance-free and lower-cost approach to removing the cooler plasma zone.
  • Torch/torch mount -A unique, demountable torch design using one-piece quartz tubing for plasma and auxiliary gas flow is supplied. The standard torch includes a 2.0-mm i.d. alumina injector for full corrosion resistance to all acids, including hydrofluoric and aqua regia. A variety of other injectors is available. The externally mounted spray chamber is integrated into an easily removed sample-introduction cassette in an enclosed compartment. The sample-introduction cassette can be adjusted (with the plasma on) for maximum performance in different matrices. No tools are required for torch or sample-introduction cassette removal.
  • Spray chambers- Instruments can be ordered with a Ryton® HF-resistant Scott-type or a glass cyclonic spray chamber. An optional spray-chamber compartment thermostat is available for laboratories with minimal air conditioning.
  • Nebulizers- The Optima 8000 can be ordered with a cross-flow or glass concentric nebulizer. The cross-flow design with GemTips™ is corrosion-resistant (sapphire/ruby tips in a PEEK body). The system can routinely handle 50% (v/v) solutions of HCl, HNO3, H2SO4, H3PO4, 20% (v/v) HF and 30% (w/v) NaOH. Additional nebulizers are available.
  • Peristaltic pump- The integrated three-channel, computer-controlled pump has variable speeds from 0.2 to 5 mL/min in 0.1 mL/min increments, using 0.76 mm (0.030 in.) i.d. tubing. Software features include FastPump™ and SmartRinse™, which dramatically improve the sample rinse-out and analysis times.
  • Power- One 200-254 VAC, 20A line (12A draw at 230 V), single phase, 50/60 Hz (±1%)
  • Dimensions- 132 x 81 x 76 cm (W x H x D), 132.5 kg
  • Environmental- The instrument will operate with a laboratory temperature between 15 and 35 °C (59-95 °F). For optimum instrument performance, the room temperature should be controlled at 20 ±2 °C.

 

Liquid Chromatography-Mass Spectrometry (LC-MS-MS)

Model: 6460 Triple Quad LC/MS & 1290 Infinity DAD

Make: Agilent Technologies

Features

Mass Spectrometer:

  • This LC/MS system features the Agilent Jet Stream technology that uses super-heated nitrogen to reduce noise and boost signals, for enhanced sensitivity and improved ion generation and desolvation.
  • Utilizes a triggered MRM (tMRM) to deliver an accurate quantitation of analytes present, even at extremely low levels, while fast polarity switching between positive and negative ions enables fast separation and high high-throughput multi-analyte studies.
  • The 6460 QQQ also has dynamic MRM to help maximize efficiency by grouping MRMs in retention time windows instead of time segments, allowing users to quantify up to 4000 compounds in a single run to maximize up time.
  • It provides 1 ms dwell time with no collision cell cross talk, and achieves sensitivity and resolution specifications with autotune.
  • The enhanced sensitivity of this triple quad system makes it an ideal choice for a broad range of applications such as clinical research, and peptide quantitation, as well as environmental and food safety analysis.
  • The 1290 Infinity Diode Array Detector (DAD) features a completely new optical design based on the Agilent Max-Light cartridge cell with optofluidic waveguides. With typical detector noise levels of < ± 0.6 µAU/cm the revolutionary 6 cm flow cell gives up to 10 times higher sensitivity than the 1200 Series DAD and VWD. Any compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift for more reliable and precise peak integration. For ultra high productivity the 1290 Infinity DAD offers multiple wavelength and full spectral detection at sampling rates up to 160 Hz.

 

Parameter

Specification

Mass range

m/z 5 – 3,000

Polarity switching

30 ms

Mass stability

< 0.1 Da in 24 h

Dynamic range

> 6.0 × 106

Scan modes

MS scan, MS/MS product ion scan, MRM, MS/MS neutral loss/gain scan and precursor ion scan, SIM

Maximum scan rate

12,500 Da/s

Minimum MRM dwell time

1 ms

MRM transitions

450 per time segment > 40,000 ion transactions per method

Dynamic MRM transitions

4,000 ion transitions per method

Triggered MRM transitions

Up to 10 MRM transitions (primary and secondary) for library search and compound confirmation

Collision cell ion clearance

< 1 ms

Autotune

Automated optimization of ion optics and mass axis calibration in positive and negative ion modes using a proprietary tune solution

Detector

High-energy conversion dynode and high-gain electron multiplier horn

Vacuum system

Two turbomolecular pumps with one mechanical pump

 

Diode Array Detector:

 

  • The 1290 Infinity Diode Array Detector (DAD) features a completely new optical design based on the Agilent Max-Light cartridge cell with optofluidic waveguides. With typical detector noise levels of < ± 0.6 µAU/cm the revolutionary 6 cm flow cell gives up to 10 times higher sensitivity than the 1200 Series DAD and VWD. Any compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift for more reliable and precise peak integration. For ultra-high productivity, the 1290 Infinity DAD offers multiple wavelength and full spectral detection at sampling rates up to 160 Hz.
  • Ultra sensitivity through revolutionary Agilent Max-Light cartridge cell with 60 mm optical path length (typically noise: < ± 0.6 µAU/cm).
  • Universal Agilent Max-Light cartridge standard cell with 10 mm optical path length provides high sensitivity (noise: < ± 3 µAU) and lowest peak dispersion for 2.1, 3 and 4.6 mm ID columns.
  • Programmable slit from 1 to 8 nm provides optimum incident light conditions for rapid optimization of sensitivity, linearity and spectral resolution.
  • Multiple wavelength and full spectral detection at high sampling rate of 160 Hz, keeping pace with fastest possible analysis speed.
  • More reliable and robust peak integration process due through less baseline drift.
  • Full spectral detection for compound identification by spectral libraries or verification of the separation quality with peak purity analysis for ultra-fast LC. Simultaneous detection of up to 8 signals for increased sensitivity and selectivity.
  • Wide linear range (typically up to 2.5 AU) – for reliable, simultaneous quantification of primary compounds, by-products and impurities.

 

Parameter

Specification

Settable flow range

0.001 – 5 mL/min, in 0.001 mL/min increments (Executed in 300pl/step increments)

Flow precision

0.07 % RSD or 0.005 min SD, whatever is greater (0.2-5.0 mL/min). (Based on retention time at constant room temperature)

Flow accuracy

±1% or 10 µL/min, whatever is greater. (Measured with water)

Maximum operating pressure

1200 bar up to 2 ml/min 800 bar up to 5 ml/min


 

Nuclear Magnetic Resonance(NMR)

Model: AVANCE-III HD 700MHz FT-NMR

Make: Bruker Biospin

Features

  • AscendTM Superconducting Magnet System equipped with 3 channel
  • Operational field- 16.4 Tesla
  • High performance vibration damping unit.
  • Electromagnetic Disturbance Suppression EDS> 99 %.
  • Bruker high performance shim system (BOSS3) with 36 orthogonal shim gradients.
  • Frequency range for each of 3 channels is 6-1020 MHz.
  • BLAXH2H amplifiers
  • Equipped with cryoplatform.
  • TCI Triple Inverse Cryoprobe for 5 mm sample tubes.
  • TXI Triple Resonance RT probe for 5 mm sample tubes.
  • Topspin 3.5 v 7

 

Matrix Assisted Laser Desorption- Time of Flight (MALDI-TOF-TOF)

Model: MALDI-TOF-TOF Ultraflextreme

Make: Bruker

Features

  • The innovative smart beam-II laser enables ultra-high acquisition speed both in MS and MS/MS at full systems performance.
  • High throughput analysis with 1 kHz laser and 384 well sample target.
  •  Digitizer provides unmatched mass resolving power Up to 40,000 mass resolution and 1 ppm mass accuracy enables precision proteomics.
  • The novel and unique laser-irradiation self-cleaning Ion Source ensures robust, long-term highest-performance operation.
  • Latest TOF/TOF technology with high efficiency and sensitivity. Typically, full MS/MS data sets can be acquired with up to 1000 Hz laser repetition rate from low fmol levels within seconds.
  • Helps top-down protein sequencing without enzymatic digestion.
  • Mascot is a powerful search engine which uses mass spectrometry data to identify proteins from primary sequence databases.

 

High Performance Thin Layer Chromatography (HPTLC)

Model: High Performance Thin Layer Chromatography (HPTLC)

Make: CAMAG

Features

High-Performance Thin-Layer Chromatography (HPTLC) is the most advanced form of TLC and comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software-controlled evaluation. HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. HPTLC meets all quality requirements of today’s analytical labs, even in a fully regulated environment. As opposed to other separation techniques, the inital costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low. The possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC. It reaches a completely new dimension in HPTLC through the use of state-of-the-art techniques for generating and evaluating digital images.

Advanced HPTLC System consist of the following:

  • CAMAG AUTOMATIC TLC SAMPLER 4 (ATS 4): Automatic sample application is a key factor for productivity of the HPTLC laboratory. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput in mass analysis.
  • CAMAG AUTOMATIC DEVELOPING CHAMBER 2 (ADC 2): CAMAG Automatic Developing Chamber 2 (ADC 2) offers convenience, safety and reproducibility for isocratic developments of TLC/ HPTLC plates and foils with the format 20 x 10 cm. The CAMAG Automatic Developing Chamber 2 (ADC 2) is the heart of an HPTLC system. It performs the development step fully automatically, reproducibly, and independent of environmental effects. The activity and pre-conditioning of the layer, chamber saturation, developing distance and final drying can be preset and automatically monitored by the ADC 2. Two modes of operation are possible: stand-alone with input of parameters via keypad, or remote operation from winCATS with process monitoring, documentation of operating parameters, and reporting.
  • TLC Scanner 4:The CAMAG TLC Scanner 4 is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects.
  • TLC Visualizer :Professional imaging and documentation system for TLC/HPTLC chromatograms with a new digital CCD camera.

 

MALDI Biotyper

Model: MALDI-TOF microflex

Make: Bruker

Features

  • Identifying microorganisms by their molecular fingerprint The MALDI Biotyper identifies microorganisms using MALDI-TOF (Matrix-Assisted Laser Desorption Ionization/Time of Flight) Mass Spectrometry to determine the unique proteomic fingerprint of an organism. The characteristic spectrum pattern of this proteomic fingerprint is used to reliably and accurately identify a particular microorganism by matching thousands of reference spectra from microorganism strains.
  • The integrated library of the MBT Software comprises spectra of thousands of strains including bacteria and fungi (yeast and mold).
  • The MBT “smart” system, the first microbiology mass spectrometry system with smartbeam™ lifetime* laser technology for increased speed.
  • High run-to-run reproducibility: The quick and simple Bacterial Test Standard quality check performed before each run provides the highest standard of reproducibility.
  • Continuous high performance: The self-cleaning MALDI Perpetual ion source permits continuous high performance with minimized maintenance requirements. The integrated IR-laser allows ion source cleaning within 15 minutes in a push-button process, decreasing downtime due to tedious manual cleaning procedures.

CHNS Analyzer

Model: 2400-II

Make: Perkin Elmer

Features

  • The 2400 Series II offers multiple analysis options: CHN, CHNS or Oxygen mode. As a user, you may choose one or more options to meet your laboratory’s needs. Changeover to different modes of operation only requires a few simple steps. The optional Column Switching Accessory (CSA) makes switching to the Oxygen mode very convenient.
  • The CHN mode is the most widely used of the analysis modes. A range of reagents and the ability to optimize the combustion parameters offer flexibility for analyzing virtually any sample types. Interfering elements such as halogens and sulfur are removed before detection.
  • The CHNS mode is specifically designed to simultaneously determine carbon, hydrogen, nitrogen and sulfur in organic materials.
  • The Oxygen mode is optimized for the automatic determination of oxygen in organic materials by pyrolyzing the sample.
  • Operating gases -In the CHN and CHNS modes, operating gases include oxygen, for combustion of sample materials, and a carrier gas – either helium or argon. The use of argon as an optional carrier gas is unique to this design and assures cost-effective use of the Elemental Analyzer in those areas of the world where helium is difficult to obtain due to price or availability.
  • In the Oxygen mode, the operating gas is helium when using silver vials or helium/hydrogen mixture when using tin vials.