Genetic Analyzers

Model: ABI 3130xl Genetic Analyzers

Make: Applied Biosystems

Features

• Auto loading of samples performed from two microtiter plates; either 96- or 384-well format
• Arrays with sixteen capillaries in four different lengths: 22, 36, 50, and 80 cm.
• Automated delivery system for POP-4, POP-6, or POP-7 polymers
• Argon laser with primary excitations at 488 and 515 nm
• Detection of up to 5 dyes for sequencing and fragment analysis
• CCD detection technology and spectrograph for color separation
• Simultaneous dual-side illumination detection system to maximize signal uniformity and sensitivity
• Software for DNA sequencing/base calling (Sequencing Analysis v5.2) and for fragment analysis of microsatellites, SNP, AFLP and LOH (GeneMapper® v4.0) as well as comparative sequencing, mutation detection, and detection of heterozygote insertions and deletions (SeqScape® v2.5).

Flow Cytometer (FCM)

Model: FACS Aria III Flow Cytometer

Make: Becton Dickinson (USA)

Features

1. Two excitation lasers:

• Blue (488 nm) Coherent Sapphire solid state; 13mW- 20mW
• Red (633 nm) JDS Uniphase He-Ne air cooled; 10mW-20mW

2. 10 measurement channels with following filters:

• FSC, 488/10
• SSC,  488/10
• FL1,  FITC,  530/30, 502LP
• FL2,  PE,  585/42, 556LP
• FL3,  PE-Texas Red, 616/23, 610LP
• FL4,  PerCP-Cy5-5,  695/40, 655LP
• FL5,  PE-Cy7 780/60 ,735LP
• FL6,  APC , 660/20
• FL7,  APC-H7,  730/45, 685LP
• FL8,  APC-Cy7,  780/60, 755LP

3. Cell Sorting:
   • Sort up to four populations simultaneously
   • Sort rates up to 25,000 cells per second
   • Sort purities > 98%
4. The sorted cells are collected in a variety tubes

• 15ml flacon tubes for 2-way sort
• 5ml FACS tubes for 4-way sort
• 1.5ml and 1.0ml microcentrifuge tubes
• 96-well plates
• Microscope slides

5. Data Analysis

• FACSDiva software Version 6.1.3 is used in FACS Aria III for operating the instrument and analyzing the data