Cellular and Molecular Biology

Flow Cytometer (FCM)

Make: Becton Dickinson (USA)

Model: FACS Aria III Flow Cytometer

Feature and uses:

FCM

  • FACS Aria III Flow Cytometer: is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles.

  • Laser configurations:

  • FSC

  • SSC

  • Blue (Wavelength 488 nm):

  • FITC: 530/30, 502 LP

  • PE: 585/42, 556 LP

  • PE-Texas Red: 616/23, 610 LP

  • PE-Cy7: 780/60, 735 LP

  • PerCP-Cy5.5: 695/40, 655 LP

  • Red (Wavelength 633 nm):

  • APC: 660/20

  • Alexa flura700: 730/45, 685 LP

  • APC-Cy7: 780/60, 755 LP

  • Violet (Wavelength 375/405 nm):

  • DAPI: 450/40

  •  Amcyan: 510/50,502 LP               

  • Cell Sorting:

  • Sort up to four populations simultaneously

  • Sort rates up to 25,000 cells/second

  • Sort purities > 98%

  • Sorted cells collected in various tubes: 5ml FACS tubes (4-way sort), 1.5ml and 1.0ml microcentrifuge tubes, 96-well plates, microscope slides

  • Data Analysis:

  • FACSDiva software Version 6.1.3 for instrument operation and data analysis

For Detailed info:Flow Cytometer Infor...

Matrix Assisted Laser Desorption Ionization - Time of Flight

Make: Bruker

Model: microflex MALDI-TOF(MALDI Biotyper)

Feature and uses:

MATRIX

·  Microorganism Identification: Uses MALDI-TOF Mass Spectrometry to determine unique proteomic fingerprints for reliable identification.

·  Extensive Library: Includes spectra of thousands of bacteria strains.

·  Smart System: Features smartbeam™ lifetime laser technology for increased speed.

·  High Reproducibility: Bacterial Test Standard quality check ensures reproducibility.

·  Continuous High Performance: Self-cleaning ion source with integrated IR-laser reduces downtime and maintenance needs.

 

For Detailed info:MALDI Biotyper BT In...